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常规内切酶 |
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| 产品编号 | 名称 | 识别位点 | 酶切位点 |
| B600257-0001 | ApaLI | GTGCAC | 5'-G↓TGCAC-3' |
| B600256-0001 | AvaIII | ATGCAT | 5′-ATGCA↓T-3′ |
| B600166-0002 | DraI | TTTAAA | 5'-TTT↓AAA-3' |
| B600259-0500 | EagI | CGGCCG | 5'-C↓GGCCG-3' |
| B600168-0005 | EcoRI | GAATTC | 5'-G↓AATTC-3' |
| B600170-0002 | EcoRV | GATATC | 5'-GAT↓ATC-3' |
| B600184-0002 | HindIII | AAGCTT | 5'-A↓AGCTT-3' |
| B600190-0001 | KpnI | GGTACC | 5′-GGTAC↓C-3′ |
| B600158-0200 | NcoI | CCATGG | 5'-C↓CATGG-3' |
| B600120-0500 | NdeI | CATATG | 5'-CA↓TATG-3' |
| B600174-0200 | NheI | GCTAGC | 5'-G↓CTAGC-3' |
| B600220-0003 | PstI | CTGCAG | 5'-CTGCA↓G-3' |
| B600226-0002 | SalI | GTCGAC | 5'-G↓TCGAC-3' |
| B600288-0500 | SmaI | CCCGGG | 5'-CCC↓GGG-3' |
| B600152-0250 | SspI | AATATT | 5'-AAT↓ATT-3' |
| B600216-0500 | StuI | AGGCCT | 5'-AGG↓CCT-3' |
| B600252-0002 | XbaI | TCTAGA | 5'-T↓CTAGA-3' |
| B600236-0002 | XhoI | CTCGAG | 5'-C↓TCGAG-3' |
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快速限制性内切酶 |
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| 产品编号 | 名称 | 识别位点 | 酶切位点 |
| B600727-0150 | SpeedyCut ApaLI | GTGCAC | 5′-G↓TGCAC-3′ |
| B600728-0050 | SpeedyCut AscI | GGCGCGCC | 5'-GG↓CGCGCC-3' |
| B600729-0500 | SpeedyCut BamHI | GGATCC | 5'-G↓GATCC-3' |
| B600730-0125 | SpeedyCut BclI | TGATCA | 5'-T↓GATCA-3' |
| B600731-0100 | SpeedyCut BglII | AGATCT | 5'-A↓GATCT-3' |
| B600732-0025 | SpeedyCut BsmBI | CGTCTC或 GAGACG |
5'-CGTCTC(N)1↓-3' 3'-GCAGAG(N)5↑-5' |
| B600733-0100 | SpeedyCut BstEII | GGTNACC | 5'-G↓GTNACC-3' |
| B600734-0050 | SpeedyCut ClaI | ATCGAT | 5'-AT↓CGAT-3' |
| B600735-0050 | SpeedyCut DpnI | Gm6ATC | 5'-Gm6A↓TC-3' |
| B600736-0050 | SpeedyCut DpnII | GATC | 5'-↓GATC-3' |
| B600737-0025 | SpeedyCut EagI | CGGCCG | 5'-C↓GGCCG-3' |
| B600738-0600 | SpeedyCut EcoRI | GAATTG | 5'-G↓AATTG-3' |
| B600739-0200 | SpeedyCut EcoRV | GATATC | 5'-GAT↓ATC-3' |
| B600740-0050 | SpeedyCut FspI | TGCGCA | 5'-TGC↓GCA-3' |
| B600741-0500 | SpeedyCut HindIII | AAGCTT | 5'-A↓AGCTT-3' |
| B600742-0500 | SpeedyCut HinfI | GANTC | 5'-G↓ANTC-3' |
| B600743-0050 | SpeedyCut HpaI | GTTAAC | 5'-GTT↓AAC-3' |
| B600744-0200 | SpeedyCut KpnI | GGTACC | 5'-GGTAC↓C-3' |
| B600745-0100 | SpeedyCut MluI | ACGCGT | 5'-A↓CGCGT-3' |
| B600746-0050 | SpeedyCut MnlI | CCTC或 GAGG |
5'-CCTC(N)7↓-3' 3'-GGAG(N)6↑-5' |
| B600747-0030 | SpeedyCut NcoI | CCATGG | 5'-C↓CATGG-3' |
| B600748-0200 | SpeedyCut NdeI | CATATG | 5'-CA↓TATG-3' |
| B600749-0030 | SpeedyCut NheI | GCTAGC | 5'-G↓CTAGC-3' |
| B600750-0050 | SpeedyCut NotI | GCGGCCGC | 5'-GC↓GGCCGC-3' |
| B600751-0100 | SpeedyCut NspV | TTCGAA | 5'-TT↓CGAA-3' |
| B600752-0050 | SpeedyCut NruI | TCGCGA | 5'-TCG↓CGA-3' |
| B600753-0200 | SpeedyCut PvuII | CAGCTG | 5'-CAG↓CTG-3' |
| B600754-0025 | SpeedyCut PacI | TTAATTAA | 5'-TTAAT↓TAA-3' |
| B600755-0500 | SpeedyCut PstI | CTGCAG | 5'-CTGCA↓G-3' |
| B600756-0100 | SpeedyCut SacI | GAGCTC | 5'-GAGCT↓C-3' |
| B600757-0200 | SpeedyCut SalI | GTCGAC | 5'-G↓TCGAC-3' |
| B600758-0060 | SpeedyCut SacII | CCGCGG | 5'-CCGC↓GG-3' |
| B600759-0025 | SpeedyCut SpeI | ACTAGT | 5'-A↓CTAGT-3' |
| B600760-0025 | SpeedyCut SbfI | CCTGCAGG | 5'-CCTGCA↓GG-3' |
| B600761-0100 | SpeedyCut SmaI | CCCGGG | 5'-CCC↓GGG-3' |
| B600762-0050 | SpeedyCut SphI | GCATGC | 5'-GCATG↓C-3' |
| B600763-0060 | SpeedyCut SspI | AATATT | 5'-AAT↓ATT-3' |
| B600764-0100 | SpeedyCut StuI | AGGCCT | 5'-AGG↓CCT-3' |
| B600765-0200 | SpeedyCut TaqI | TCGA | 5'-T↓CGA-3' |
| B600766-0500 | SpeedyCut XbaI | TCTAGA | 5'-T↓CTAGA-3' |
| B600767-0500 | SpeedyCut XhoI | CTCGAG | 5'-C↓TCGAG-3' |
生工生物限制性内切酶分为两种：一类是常规的限制性内切酶，一类为快切的限制性内切酶，主要区别为酶切的速度。
常规的限制性内切酶酶切时间一般在2 h左右，里面附带的10× Single Buffer，在普通限制性内切酶进行双酶切时可以作为酶切buffer使用；
SpeedyCut 快切的限制性内切酶一般在15~30 min左右，在反应时间上大大缩短，节省了实验时间。所有的限制酶都适用于附带的 SpeedyOne 缓冲液，可在统一反应体系内任意组合双酶切，无需多次单酶切或更换酶切缓冲液。
注:进行双酶切时建议用同一公司同一系列的限制性内切酶，以防因公司或产品系列的不同双酶切困难