概述

FOCUS(TM) Global Fractionation kit is specifically designed for a simple, rapid and highly reproducible method for fractionation of various classes of cellular proteins. This kit allows fractionation of soluble and insoluble proteins. The insoluble protein fraction may be further fractionated into various classes of membrane proteins, such as mildly hydrophobic membrane proteins, highly hydrophobic trans‐membrane proteins, and membrane proteins rich in cholesterol, glycolipids, and glycosyl‐phosphitidylinositol (GPI).

特点

• Fractionate integral, peripheral and lipid raft associated proteins;
• Fractionation of complex proteomes into multiple, specific fractions.

应用

Selective fractionation of membrane proteins from tissues, cells, plants, yeast, bacteria, insects, and other sources; Fractionation of membrane proteins from lipid rafts; Suitable for membrane protein preparation for 2D gel electrophoresis and other applications; Suitable for proteomics and cell signaling studies.

组分

SPE Buffer; MPE Buffer; MSE Buffer; FPS Buffer; DILUENT‐III