概述

All live cells and tissues contain proteases, which are liberated during the processing of samples. Sensitive protease assays are needed to study protease activity present in the sample of interest. G‐Biosciences Protease Assay Kit is designed for the quantitative determination of proteases present in the protein sample, and uses a dye‐ labeled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labeled peptides. The absorbance of the dye‐labeled peptide is measured at 570nm for determination of protease activity. Chemically stabilized Trypsin is supplied with the kit as a general protease standard; however, other specific protease standard can also be used.

应用

The proteases present in the sample of interest will digest the protein substrate and release dye labeled peptides. The absorbance of the dye-labeled peptide is measured at 570 nm for determination of protease activity.

组分

Incubation Buffer; Precipitation Agent; Assay Buffer; Protease Substrate (lyophilized);Trypsin, Mass Spectrometry Grade (bovine); Trypsin Suspension Buffer

属性

ITEMS NEEDED BUT NOT SUPPLIED

96 well titer plates