DSS is a water-insoluble, homobifunctional N-hydroxysuccinimide ester (NHS ester),NHS esters react efficiently with primary amino groups (-NH2) in pH7-9 buffers to form stable amide bonds. The reaction results in the release of N-hydroxysuccinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester reagents.
DSS is hydrophobic and dissolved in an organic solvent such as DMSO or DMF then added to the aqueous reaction mixture. DSS does not possess a charged group and is lipophilic and membrane-permeable, which makes it useful for intracellular and intramembrane conjugations. Water-soluble BS3 possess a charged group and is useful for cell-surface protein crosslinking.
100 mg/bottle or 500 mg/bottle
|>95% on TLC