概述

SATA is the reagentfor introducing protected sulfhydryls into proteins, peptides and other molecules. They are the N-hydroxysuccinimide (NHS) esters of S-acetylthioacetic and propionic acid. A stable, covalent amide bond is formed from the reaction of the NHS ester with primary amines. The amine reacts with the NHS ester by nucleophilic attack, with N-hydroxysuccinimide being released as a by-product. Deprotection (deacylation) to generate a sulfhydryl for use in cross-linking and other applications is accomplished using hydroxylamine HCl.

特点

• Reaction conditions are mild and non-denaturing;The reaction is specific toward primary amines;Sulfhydryl groups are introduced in a protected form, allowing the modified molecule to be stored indefinitely and then later treated with hydroxylamine to expose the labile sulfhydryl group for final conjugation reactions.

应用

Soluble in DMSO

组分

100 mg/bottle

属性

Molecular Weight	231.23
Spacer Arm Length	2.8
Purity	>95% on TLC
Reactive groups	NHS ester and thioacetyl protected sulfhydryl