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Order NO. D110005
兔抗FLAG Tag多克隆抗体
英文名 Anti-FLAG Tag rabbit polyclonal antibody
别名 DDDDK epitope tag; DYKDDDDK epitope tag
相关类别 标签抗体 储存 冷冻（-20℃）
宿主 Rabbit 反应种属 Recombinant proteins containing the FLAG Tag
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 FLAG Tag    
编 号 规格 库存 目录价（￥） 您的价格（￥） 数 量
D110005-0025 25 ul 现货 224 224
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D110005-0100 100 ul 现货 525 525
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D110005-0200 200 ul 现货 840 840
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属性

Background: The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB
Name of antibody: FLAG Tag
Immunogen: Synthetic peptide: 3*DYKDDDDK conjugated to KLH
Full name: FLAG Tag
Synonyms: DDDDK epitope tag; DYKDDDDK epitope tag
ELISA Recommended dilution: 5000-10000
WB Predicted band size: 50/40 kDa
WB Positive control: Recombinant proteins containing the Flag Tag
WB Recommended dilution: 10000-50000
相关文档
Sample Application

  • Gel: 8%SDS-PAGE, Lysate: 0.1/1 μg, Lane 1-2: 0.1/1 μg Recombinant proteins containing the Flag Tag, Primary antibody: D110005(FLAG Tag Antibody) at dilution 1/10000, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/20000 dilution, Exposure time: 30 seconds