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Order NO. D124146
兔抗UBE2M多克隆抗体
英文名 Anti-UBE2M rabbit polyclonal antibody
别名 UBC12; hUbc12; UBC-RS2
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 UBE2M    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D124146-0025 25 ul 现货
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D124146-0100 100 ul 现货
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D124146-0200 200 ul 现货
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属性

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008] (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: UBE2M
Immunogen: Recombinant protein of human UBE2M
Full name: ubiquitin conjugating enzyme E2 M
Synonyms: UBC12; hUbc12; UBC-RS2
SwissProt: P61081
ELISA Recommended dilution: 5000-10000
IHC positive control: Human thyroid cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 21 kDa
WB Positive control: A549, LO2 cell lysates
WB Recommended dilution: 1000-5000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using D124146(UBE2M Antibody) at dilution 1/70, on the right is treated with recombinant protein. (Original magnification: ×200)


  • Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A549, LO2 cell lysates, Primary antibody: D124146(UBE2M Antibody) at dilution 1/1000, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 1 minute
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