兔抗UBE2M多克隆抗体,Anti-UBE2M rabbit polyclonal antibody 生命科学产品与技术服务-生工生物工程(上海)股份有限公司

Order NO. D124146

兔抗UBE2M多克隆抗体

英文名	Anti-UBE2M rabbit polyclonal antibody
别名	UBC12; hUbc12; UBC-RS2
相关类别	一抗	储存	冷冻（-20℃）
宿主	Rabbit	反应种属	Human, Mouse
标记物	Unconjugate	克隆类型	rabbit polyclonal
抗原	UBE2M
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属性

Background:	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008] (The background information is obtained from official databases and does not represent the product information.)
Applications:	ELISA, WB, IHC
Name of antibody:	UBE2M
Immunogen:	Recombinant protein of human UBE2M
Full name:	ubiquitin conjugating enzyme E2 M
Synonyms:	UBC12; hUbc12; UBC-RS2
SwissProt:	P61081
ELISA Recommended dilution:	5000-10000
IHC positive control:	Human thyroid cancer
IHC Recommend dilution:	50-200
WB Predicted band size:	21 kDa
WB Positive control:	A549, LO2 cell lysates
WB Recommended dilution:	1000-5000

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Sample Application

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using D124146(UBE2M Antibody) at dilution 1/70, on the right is treated with recombinant protein. (Original magnification: ×200)
Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A549, LO2 cell lysates, Primary antibody: D124146(UBE2M Antibody) at dilution 1/1000, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 1 minute