小鼠抗6X His Tag单克隆抗体,Anti-6X His Tag mouse monoclonal antibody 生命科学产品与技术服务-生工生物工程(上海)股份有限公司

Order NO. D191001

小鼠抗6X His Tag单克隆抗体

英文名	Anti-6X His Tag mouse monoclonal antibody
别名	6 His epitope tag
相关类别	标签抗体	储存	冷冻（-20℃）避光
宿主	Mouse	反应种属	Fusion proteins containing the His Tag
克隆类型	mouse monoclonal	抗原	6xHis Tag
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编号	规格	库存	目录价（￥）	您的价格（￥）	数量
D191001-0025	25 ul	现货	224	224
D191001-0100	100 ul	现货	525	525
D191001-0200	200 ul	现货	840	840

产品描述

概述

The 6 X histidine motif is used as a Tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-Tag fused to targeted proteins in transfected or transformed cells.

应用

免疫印迹（WB），免疫荧光（IF），免疫沉淀（IP）

属性

保存缓冲液	Ascitic fluid with 0.05% sodium azide and 40% glycerol
运输条件	2-8°C
靶点全称	HHHHHH Tag
靶点别名	6 His epitope Tag
靶点研究领域	Cell Biology
亚型	IgG1

相关文档

Sample Application

Gel: 8%SDS-PAGE, Lysate: 2 μg, Lane: Fusion proteins containing the His Tag, Primary antibody: D191001(6×His Tag Antibody) at dilution 1/3000, Secondary antibody: Goat anti mouse IgG at 1/5000 dilution, Exposure time: 1 minute
The image is immunofluorescence of methanol-fixed 3T3 cells transfected with His-TAF4B vector using D191001(6×His Tag Antibody) at dilution 1/500
Immunoprecipitation analysis: Lane 1 is Transfected cell lysate(Input), Lane 2 is Transfected cell lysate using D191001(6×His Tag Antibody) at dilution 1/200, Lane 3 is Normal Mouse IgG(Control)