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Order NO. D223663
兔抗RPL35A多克隆抗体
英文名 Anti-RPL35A rabbit polyclonal antibody
别名 DBA5; L35A
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 RPL35A    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D223663-0025 25 ul 现货
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D223663-0100 100 ul 现货
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D223663-0200 200 ul 现货
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属性

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Oct 2015] (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: RPL35A
Immunogen: Fusion protein of human RPL35A
Full name: ribosomal protein L35a
Synonyms: DBA5; L35A; eL33
SwissProt: P18077
ELISA Recommended dilution: 5000-10000
IHC positive control: Human liver cancer and Human lung cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 13 kDa
WB Positive control: Jurkat cell lysate
WB Recommended dilution: 500-2000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using D223663(RPL35A Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using D223663(RPL35A Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)


  • Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Jurkat cell lysate, Primary antibody: D223663(RPL35A Antibody) at dilution 1/700, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 10 seconds
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