ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]
Applications:
WB
Name of antibody:
ACLY (phospho-Thr447/Ser451)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr447/Ser451 of human ACLY
Full name:
ATP citrate lyase
Synonyms:
ACL; ATPCL; CLATP
SwissProt:
P53396
WB Predicted band size:
121 kDa
WB Positive control:
K562 lysate
WB Recommended dilution:
500-2000
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Sample Application
Gel: 6%SDS-PAGE, Lysate: 30 μg, Lane: K562 lysate, Primary antibody: D290018(ACLY (phospho-Thr447/Ser451) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes