Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Applications:
WB
Name of antibody:
GRIA1 (phospho-Ser845)
WB Recommended dilution:
500-2000
WB Positive control:
U87-MG, C6 lysates
WB Predicted band size:
102 kDa
SwissProt:
P42261
Synonyms:
GLUH1; GLUR1; GLURA; GluA1; HBGR1; MRD67; MRT76
Full name:
glutamate ionotropic receptor AMPA type subunit 1
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Ser845 of human GRIA1
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Sample Application
Gel: 6%SDS-PAGE, Lysate: 30 μg, Lane 1-2: U87-MG, C6 lysates, Primary antibody: D290600(GRIA1 (phospho-Ser845) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes