Enables ATP binding activity and protein serine/threonine kinase activity. Involved in several processes, including histone-serine phosphorylation; positive regulation of histone modification; and regulation of transcription, DNA-templated. Located in cytoplasm and nucleoplasm. [provided by Alliance of Genome Resources, Apr 2022]
Applications:
WB, IF
Name of antibody:
RPS6KA5 (phospho-Ser360)
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Ser360 of human RPS6KA5
Full name:
ribosomal protein S6 kinase A5
Synonyms:
MSK1; RLPK; MSPK1
SwissProt:
O75582
WB Predicted band size:
90 kDa
WB Positive control:
C6, CHO-K1 lysates
WB Recommended dilution:
500-2000
IF Positive control:
Hela cells
IF Recommended dilution:
50-200
相关文档
Sample Application
The image is immunofluorescence of Hela cells using D291359(RPS6KA5 (phospho-Ser360) Antibody) at dilution 1/50 (green). Blue: DAPI
Gel: 6%SDS-PAGE, Lysate: 30 μg, Lane 1-2: C6, CHO-K1 lysates, Primary antibody: D291359(RPS6KA5 (phospho-Ser360) Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes