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Order NO. D291648
兔抗VIM单克隆抗体
英文名 Anti-VIM rabbit monoclonal antibody
别名 vimentin
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat, Hamster
标记物 Unconjugate 克隆类型 rabbit monoclonal
抗原 VIM    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D291648-0050 50 ul 预计5日内发货
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D291648-0100 100 ul 预计5日内发货
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属性

Background: This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Applications: WB, IHC, IF
Name of antibody: VIM
Immunogen: Synthetic peptide of human VIM
Full name: vimentin
SwissProt: P08670
IHC positive control: Human tonsil
IHC Recommend dilution: 50-100
WB Predicted band size: 54 kDa
WB Positive control: C6, CHO-K1, Hela, 3T3 lysates
WB Recommended dilution: 500-2000
IF Positive control: Hela cells
IF Recommended dilution: 50-200
相关文档
Sample Application

  • The image is immunofluorescence of Hela cells using D291648(VIM Antibody) at dilution 1/50 (green). Blue: DAPI

  • The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using D291648(VIM Antibody) at dilution 1/50. (Original magnification: ×200)

  • Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-4: C6, CHO-K1, Hela, 3T3 lysates, Primary antibody: D291648(VIM Antibody) at dilution 1/1000, Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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