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产品变更说明-E672002等
尊敬的客户:
因产品产品优化,经过公司管理层审批同意,我们将对如下产品执行信息变更。计划执行时间为2026/10/15,期间可能会出现实际交货产品变更前后两种情况共存的现象,生工生物深感歉意并希望得到您的谅解!我们承诺,本次变更不会对产品质量产生负面影响,敬请继续放心订购与使用。
| 产品编号 | 产品名称 | 变更类型 | 变更前 | 变更后 |
|---|---|---|---|---|
| E672002 | 4%多聚甲醛固定液 | 操作流程 | 产品简介 :本产品主要用于免疫染色时细胞样品或组织切片的固定。 按照每个样品固定时需要1 mL固定液计算,一个包装的免疫染色固定液可以固定100/500个样品。保存条件 :2-8°C保存,保质期至少6个月;-20°C保存,保质期至少12个月。 操作步骤 :1. 对于细胞样品,去除培养液后,六孔板中每孔加入1 mL固定液。 2.对于组织样品,加入免疫染色固定液的量均以充分覆盖样品为准。 3. 固定时间:通常室温固定10 min即可完成固定,亦可4°C固定过夜。根据样品材料、大小不同,固定时间可能会有较大差异。 | 产品简介:本产品为即用型4%多聚甲醛(Paraformaldehyde, PFA)固定液,以磷酸盐缓冲液为溶剂配制,适用于组织切片、细胞爬片、免疫组织化学、免疫荧光及流式细胞术等实验中的样本固定。多聚甲醛是一种常用的交联固定剂,可通过形成亚甲基桥共价交联蛋白质中的氨基基团,从而稳定细胞和组织结构,良好地保存抗原性和细胞形态。保存条件:-20°C保存,保质期12个月。操作步骤:1.组织固定:室温或4°C浸泡2~24 h,根据组织大小调整固定时间。2.细胞固定:培养细胞或细胞爬片室温或4°C浸泡10~30 min。注意事项:1. 产品融化后有沉淀或浑浊时,可置37°C水浴,溶解后不影响使用。 |
| 操作流程 | Description: This product is mainly used to immobilize immunostained cell samples or tissue sections. Based on 1 mL of fixative required for each sample immobilization, 100/500 samples can be mounted in a single, immunostaining fixative. Storage: Store at 2-8°C for at least 6 months; Store at -20°C for at least 12 months. Procedure: 1. For cell samples, remove the culture and add 1 mL of fixative to each well of the six-well plate. 2. For tissue samples, add the amount of immunostaining fixative to allow adequate coverage of the tissue. 3. Fixed time: usually fixed at room temperature for 10 min to complete the fixed, but also 4°C fixed overnight. According to the sample material, the size of the fixed time may be a big difference. |
Description: This product is a ready-to-use 4% paraformaldehyde (PFA) fixative solution prepared in phosphate-buffered saline. It is suitable for sample fixation in tissue sections, cell coverslips, immunohistochemistry, immunofluorescence, and flow cytometry.Paraformaldehyde is a commonly used cross-linking fixative that covalently cross-links amino groups in proteins via methylene bridge formation, thereby stabilizing cellular and tissue structures and effectively preserving antigenicity and cell morphology.Product Features: Strong penetration ability, uniform fixation, minimal tissue shrinkage, and excellent staining results. Storage: Store at -20°C for 12 months.Procedure: 1.Tissue: RT or 4°C for 2~24 h. Adjust the fixed time according to the size of the tissue.2.Cell: RT or 4°C for 10~30 min.Precautions: 1. If there are precipitates or turbidity after the product melts, it can be placed in a 37°C water bath. The use of the product will not be affected after dissolution. |
生工生物工程(上海)股份有限公司
2026年10月15日 星期四