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Order NO. A004070
S1 核酸酶
英文名 Nuclease, S1
级别 Activity ≥100,000 to 500,000 U/ml 相关类别 生化试剂
储存 冷冻(-20℃)    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
A004070-0010 10 KU 预计90日内发货
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A004070-0050 50 KU 预计90日内发货
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产品描述

概述

Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.

属性

Source Aspergillus oryzae
Description Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.
Storage Store at -20°C.
REQUIRES SPECIAL SHIPPING ICE PACK
Activity ≥100,000 to 500,000 units per ml
Unit Definition One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.
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