Order NO. A004070
产品描述
概述
Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.
属性
Source |
Aspergillus oryzae
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Description |
Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.
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Storage |
Store at -20°C.
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REQUIRES SPECIAL SHIPPING |
ICE PACK
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Activity |
≥100,000 to 500,000 units per ml
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Unit Definition |
One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.
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