概述

HOOK Cell Surface Protein Isolation kit uses Sulfo-NHS-SS-Biotinlabeling andStreptavidinpurification technology to conveniently label cell surface proteins and isolate them for further analysis, inconjunction with our Mammalian Cell PE LB lysis buffer. Finally the biotin labeled cell surface proteins are then released by reduction of the disulfide bond with DTT.This kit is supplied with sufficient reagents for five experiments, with each experiment consisting of four 90-95% confluent T-75cm2 flasks.

特点

• Complete cell surface biotin labeling and isolation;
• Suitable for a wide selection of mammalian cells;
• Application for the isolation of cell surface proteins, the study of cell surface trafficking , the study of receptor and ligand interactions.

应用

For the isolation of cell surface proteins; For the study of cell surface trafficking; For the study of receptor:ligand interactions.

组分

HOOK-Sulfo-NHS-SS-Biotin; Streptavidin-Agrose column with stoppers; Collection Tube (2ml); Mammalian Cell PE LB; HOOK Quench Buffer; JAW Phosphate Buffered Saline Packs (1 liter); OneQuant DTT