概述

The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485nm and emission at 535nm to determine protease activity. The kit detects picogram level of proteases present in the sample.

应用

The kit components are sufficient for 1,000 assays in a microtiter plate format. Application for quantitative fluorescence protease assay.

组分

Fluoro(TM) Assay Buffer; FITC- Casein Protease Substrate (lyophilized); MSG-Trypsin – Porcine (TPCK Treated); Trypsin Suspension Buffer