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英文名 | Iodoacetyl LC Biotin | ||
别名 | N-Iodoacetyl-N-biotinylhexylenediamine | ||
相关类别 | 生物素标记试剂 | 储存 | 冷藏(2-8℃) |
分子量 | 510.4 | Uniprot ID | N-碘代乙酸基-N-生物素己二胺 |
编 号 | 包装 | 库存 | 目录价(¥) | 您的价格(¥) | 数 量 |
C100218-0050 | 50 MG | 现货 |
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概述
Iodoacetyl-LC-Biotin is long-chain, sulfhydryl-reactive biotinylation reagents.For the reagent,reaction of the iodoacety1 group with a sulfhydryl (-SH) group is rapid and specific, especially when only a slight reagent-to-sulfhydrylmolar excess is used and the reaction is performed at pH 8.3 (7.5-8.5).1-3 The reaction occurs by nucleophilic substitution of iodine with a thiol (sulfhydryl) group, resulting in a stable thioether bond (Figure 1). If thiols are unavailable on the molecule, reaction can occur with histidy1 side chains at pH 6.9-7.0, but the reaction must be allowed to proceed for at least one week. Iodoacetyl reagents also will react with amino groups at pH > 10.
应用
Iodoacetyl-LC-Biotin is not soluble in water and must be dissolved in an organic solvent such as DMF before further dilution in aqueous solutions.
组分
50 mg/bottle
属性
Formula | C18H31IN4O3S |
Net Mass Addition | 382.53 |
Spacer arm | 27.1 |
Purity | >95% on TLC |
Solubility | > 2 mg/ml in DMF for subsequent dilution in water or buffer |