![](/images/brand/BBI.png)
英文名 | Biotin BMCC | ||
别名 | (1-Biotinamido)-4-[4'-(maleimidomethyl)cyclohexanecarboxamido]hexane | ||
相关类别 | 生物素标记试剂 | 储存 | 冷藏(2-8℃) |
分子量 | 533.7 | Uniprot ID | 1-生物素酰氨基-4-[4`-(甲基马来酰胺)环己烷甲酰胺]丁烷 |
编 号 | 包装 | 库存 | 目录价(¥) | 您的价格(¥) | 数 量 |
C100222-0050 | 50 MG | 现货 |
![]() |
![]() |
![]() |
概述
Biotin-BMCC is a sulfhydryl-reactive biotinylation reagent with a long spacer arm. Maleimide-activated reagents are effective for protein modification of sulfhydryl groups. Maleimide groups react efficiently and specifically with free (reduced) sulfhydryls at pH 6.5-7.5 to form stable thioether bonds (Figure 1). Most proteins have cysteine residues whoseside-chain sulfur atoms typically occur in pairs as disulfide bonds. Reduction of these disulfide bonds exposes the sulfhydryl group required as a target for biotinylation with maleimide-activated reagents. Alternatively, sulfhydryl groups can be addedto molecules using various modification reagents (see subsequent Important Product Information Section).
应用
Biotin-BMCC is not directly soluble in water or aqueous buffers; however, if it is first dissolved in dimethylsulfoxide (DMSO) or other organic solvent, then it can be diluted and remain soluble in an aqueous buffer at typical reaction concentrations.
组分
50 mg/bottle
属性
Molecular Weight | 533.68 |
Spacer arm | 32.6 |
Purity | >95% on TCL |
Reaction pH | acidic or neutral pH |
Solubility | ~4.5 mg/ml (8.5 mM) in DMSO (slightly less soluble in DMF) |