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![](/productImage/PIC/C100333/C100333.png)
英文名 | EMCS | ||
别名 | EMCS | ||
相关类别 | 蛋白异源双功能交链试剂 | 储存 | 冷藏(2-8℃) |
分子量 | 308.3 | Uniprot ID | 6-(马来酰亚胺基)己酸琥珀酰亚胺酯 |
编 号 | 包装 | 库存 | 目录价(¥) | 您的价格(¥) | 数 量 |
C100333-0050 | 50 MG | 咨询 |
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概述
EMCS and its water-soluble analog Sulfo-EMCS are heterobifunctional cross-linkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these cross-linkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
应用
EMCS is not directly water-soluble and must be dissolved first in an organic solvent such as dimethylsulfoxide (DMSO) or dimethylformamide (DMF); subsequent dilution into aqueous reaction buffer is generally possible, and most protein reactants will remain soluble if the final concentration of organic solvent is less than 10%.
组分
50 mg/bottle
属性
Molecular Weight | 308.29 |
Spacer Arm Length | 9.4 |
Purity | >95% on TLC |