DSP is a water-insoluble, homobifunctional N-hydroxysuccimide ester (NHS-ester) and DTSSP is its water-soluble analog. These crosslinkers are thiol-cleavable, primary amine-reactive and have been used in many applications. NHS-ester reactions with primary amines form covalent amide bonds that results in the release of N-hydroxysuccinimide. DSP is non-sulfonated and, therefore, is non-water-soluble whereas DTSSP is sulfonated and water-soluble.
DSP is first dissolved in an organic solvent and added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and is useful for intracellular and intramembrane conjugation.
100 mg/bottle or 500 mg/bottle
|>95% on TLC
|NHS ester - homobifunctional cleavable