Order NO. C520462
产品描述
概述
Recombinant E.coli Beta-Glucuronidase is produced by our E.coli expression system and the target gene encoding Met1-Gln603 is expressed with a 6His tag at the N-terminus.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
属性
Tag |
N-6His
|
种属 |
E.coli
|
表达系统 |
E.coli
|
Accession# |
P05804
|
Source |
E.coli
|
Formulation_Description |
Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
|
Storage |
Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
|
Purity |
Greater than 95% as determined by reducing SDS-PAGE.
|
Endotoxin |
Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
|
Background |
Beta-D-glucuronidase from E.coli is a highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.
|