重组人FGFb (Met1-Ser155)(Q65I,C96S,N111G), None, E.coli,Recombinant Human Thermostable Fibroblast Growth Factor 2(Q65I,C96S,N111G) , None, E.coli 生命科学产品与技术服务-生工生物工程(上海)股份有限公司

Order NO. C520995

重组人FGFb (Met1-Ser155)(Q65I,C96S,N111G), None, E.coli

英文名	Recombinant Human Thermostable Fibroblast Growth Factor 2(Q65I,C96S,N111G) , None, E.coli
相关类别	其他重组蛋白	储存	冷冻（-20℃）

编号	包装	库存	目录价（￥）	您的价格（￥）	数量
C520995-0010	10 UG	预计10日内发货
C520995-0050	50 UG	预计10日内发货

产品描述

概述

Recombinant Human Thermostable Fibroblast Growth Factor 2(Q65I,C96S,N111G) is produced by our E.coli expression system and the target gene encoding Met1-Ser155 is expressed.

使用说明

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

属性

Tag	None
种属	Human
表达系统	E.coli
Accession#	P09038-2
Source	E.coli
Formulation_Description	Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH 7.5.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution	Dissolve the lyophilized protein in distilled water.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
Background	Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule.