重组人Shh (C24II), None, E.coli,Recombinant Human Sonic Hedgehog , None, E.coli 生命科学产品与技术服务-生工生物工程(上海)股份有限公司

Order NO. C521923

重组人Shh (C24II), None, E.coli

英文名	Recombinant Human Sonic Hedgehog , None, E.coli
相关类别	其他重组蛋白	储存	冷冻（-20℃）

编号	包装	库存	目录价（￥）	您的价格（￥）	数量
C521923-0010	10 UG	预计10日内发货
C521923-0050	50 UG	预计10日内发货

产品描述

概述

Recombinant Human Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys24-Gly197(Cys24Ile-Ile) is expressed.

使用说明

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

属性

Tag	None
种属	Human
表达系统	E.coli
Accession#	Q15465
Source	E.coli
Formulation_Description	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution	Dissolve the lyophilized protein in distilled water.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
Background	Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.