重组小鼠SCF, None, E.coli,Recombinant Mouse Stem Cell Factor , None, E.coli 生命科学产品与技术服务-生工生物工程(上海)股份有限公司

Order NO. C521943

重组小鼠SCF, None, E.coli

英文名	Recombinant Mouse Stem Cell Factor , None, E.coli
相关类别	其他重组蛋白	储存	冷冻（-20℃）

产品描述

概述

Recombinant Mouse Stem Cell Factor is produced by our E.coli expression system and the target gene encoding Lys26-Ala189 is expressed.

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Tag	None
种属	Mouse
表达系统	E.coli
Accession#	P20826
Source	E.coli
Formulation_Description	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Storage	Lyophilized protein should be stored at < -20°C, thoμgh stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution	Dissolve the lyophilized protein in distilled water.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
Background	Mouse stem cell factor (SCF), is the ligand for the receptor-type protein-tyrosine kinase KIT. It plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. It also promotes phosphorylation of PIK3R1, which is the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5.
Biological_Activity	Measured by the dose-dependent stimulation of TF-1 cells. The ED50 for this effect is 4-12 ng/ml.