重组大鼠TNFa, None, E.coli,Recombinant Rat Tumor Necros, None, E.coli 生命科学产品与技术服务-生工生物工程(上海)股份有限公司

Order NO. C522052

重组大鼠TNFa, None, E.coli

英文名	Recombinant Rat Tumor Necros, None, E.coli
相关类别	其他重组蛋白	储存	冷冻（-20℃）
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产品描述

概述

Recombinant Rat Tumor Necrosis Factor is produced by our E.coli expression system and the target gene encoding Leu80-Leu235 is expressed.

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Tag	None
种属	Rat
表达系统	E.coli
Accession#	P16599
Source	E.coli
Formulation_Description	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution	Dissolve the lyophilized protein in distilled water.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin	Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
Background	Tumor necrosis factor alpha (TNF-alpha, TNFSF2) is the prototypic ligand of the TNF superfamily. Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.