Order NO. C600190
产品描述
概述
CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. Murine CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. Both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, murine CXCL12α shares approximately 93 % amino acid sequence identity with human CXCL12α.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
属性
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder.
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| Purity |
> 97 % by SDS-PAGE and HPLC analyses.
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| Source |
Escherichia coli.
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| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.
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| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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| Endotoxin |
Less than 1 EU/μg of rMuSDF-1α/CXCL12α as determined by LAL method.
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| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
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| AA Sequence |
KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
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