重组小鼠NOV,Recombinant Murine Nephroblastoma-overexpressed Gene Protein Homolog 生命科学产品与技术服务-生工生物工程(上海)股份有限公司

Order NO. C600365

重组小鼠NOV

英文名	Recombinant Murine Nephroblastoma-overexpressed Gene Protein Homolog
别名	NOV, Murine
相关类别	其他重组蛋白	储存	冷冻（-20℃）
分子量	Approximately 36.4 kDa, a single non-glycosylated polypeptide chain containing 333 amino acids.
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编号	规格	库存	目录价（￥）	您的价格（￥）	数量
C600365-0005	5 UG	预计20日内发货	待询价	待询价

产品描述

概述

NOV is a member of the CCN family of secreted cysteine rich regulatory proteins. The full length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm’s tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorgenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and cell migration by signaling through specific cell surface integrins and by binding to heparin sulfate proteoglycans and to fibulin 1C. NOV has also been reported to exert proangiogenic activities. Recombinant murine NOV is a 36.4 kDa protein containing 333 amino acid residues. It is composed of four distinct structural domains (modules) - the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).

使用说明

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

属性

Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Source	Escherichia coli.
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.6, 150 mM NaCl.
Endotoxin	Less than 0.1 EU/μg of rMuNOV as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
AA Sequence	QVSASLRCPS RCPPKCPSIS PTCAPGVRSV LDGCSCCPVC ARQRGESCSE MRPCDQSSGL YCDRSADPNN QTGICMVPEG DNCVFDGVIY RNGEKFEPNC QYFCTCRDGQ IGCLPRCQLD VLLPGPDCPA PRKVAVPGEC CEKWTCGSDE QGTQGTLGGL ALPAYRPEAT VGVEVSDSSI NCIEQTTEWS ACSKSCGMGV STRVTNRNRQ CEMVKQTRLC IVRPCEQEPE EV