IHC image of β-catenin staining in a formalin fixed, paraffin embedded human breast cancer tissue (left) and colorectal cancer tissue (right). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer for 20 minutes. The tissue section was then incubated with mouse anti-β-catenin（C650043）antibody overnight at 4℃.HRP-conjugated goat anti-mouse IgG was used as secondary antibody. (Original magnification: 100×).