IHC image of CK14 staining (left) and CK18 staining (right) in a formalin fixed, paraffin embedded human Esophageal squamous cell carcinoma (left) and Appendix tissue (right). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer for 20 minutes. The tissue section was then incubated with 1 μg/ml mouse anti-CK14 antibody (C650027) and anti-CK18 (C650011) overnight at 4°C. HRP-conjugated goat anti-mouse IgG was used as secondary antibody (C650201). (Original magnification: 100X).