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Order NO. D110002
兔抗6X His Tag多克隆抗体
英文名 Anti-6X His Tag rabbit polyclonal antibody
别名 6 His epitope tag
相关类别 标签抗体 储存 冷冻（-20℃）
宿主 Rabbit 反应种属 Recombinant protein containing the HIS Tag
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 6X His Tag    
编 号 规格 库存 目录价（￥） 您的价格（￥） 数 量
D110002-0025 25 ul 现货 待询价 待询价
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D110002-0100 100 ul 现货 待询价 待询价
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D110002-0200 200 ul 现货 待询价 待询价
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属性

Background: The 6X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. (The background information is obtained from official databases and does not represent the product information.)
Applications: WB
Name of antibody: 6X His Tag
Immunogen: Synthetic peptide: 18X His conjugated to KLH
Full name: HHHHHH Tag
WB Predicted band size: 55/45/57 kDa
WB Positive control: Fusion proteins containing the His Tag
WB Recommended dilution: 2000-10000
相关文档
Sample Application

  • Gel: 8%SDS-PAGE, Lysate: 0.5 μg, Lane 1-3: Fusion proteins containing the His Tag, Primary antibody: D110002(6X His Tag Antibody) at dilution 1/4000, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 20 seconds