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Order NO. D110016
兔抗GAPDH多克隆抗体
英文名 Anti-GAPDH rabbit polyclonal antibody
别名 GAPD, G3PD, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
相关类别 内参抗体 储存 冷冻（-20℃）
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 GAPDH    
编 号 规格 库存 目录价（￥） 您的价格（￥） 数 量
D110016-0025 25 ul 现货 待询价 待询价
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D110016-0100 100 ul 现货 待询价 待询价
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D110016-0200 200 ul 现货 待询价 待询价
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属性

Background: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: GAPDH
Immunogen: Recombinant protein of human GAPDH
Full name: glyceraldehyde-3-phosphate dehydrogenase
Synonyms: G3PD; GAPD; HEL-S-162eP
SwissProt: P04406
ELISA Recommended dilution: 5000-10000
IHC positive control: Human liver cancer and Human tonsil
IHC Recommend dilution: 50-200
WB Predicted band size: 36 kDa
WB Positive control: HT-29, PC-3, K562, HepG2, Raji, RAW264.7 cell lysates
WB Recommended dilution: 5000-10000
相关文档
Sample Application

  • The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using D110016(GAPDH Antibody) at dilution 1/70. (Original magnification: ×200)

  • The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using D110016(GAPDH Antibody) at dilution 1/70. (Original magnification: ×200)

  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-6: HT-29, PC-3, K562, HepG2, Raji, RAW264.7 cell lysates, Primary antibody: D110016(GAPDH Antibody) at dilution 1/4000, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 1 second