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Order NO. D121906
兔抗SPHK2多克隆抗体
英文名 Anti-SPHK2 rabbit polyclonal antibody
别名 SK 2; SK-2; SPK 2; SPK-2
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 SPHK2    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D121906-0025 25 ul 现货
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D121906-0100 100 ul 现货
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D121906-0200 200 ul 现货
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属性

Background: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: SPHK2
Immunogen: Recombinant protein of human SPHK2
Full name: sphingosine kinase 2
Synonyms: SK 2; SK-2; SPK 2; SPK-2
SwissProt: Q9NRA0
ELISA Recommended dilution: 5000-10000
IHC positive control: Human colorectal cancer and Human cervical cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 69 kDa
WB Positive control: RAMOS, LO2 cell lysates
WB Recommended dilution: 1000-5000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using D121906(SPHK2 Antibody) at dilution 1/60, on the right is treated with recombinant protein. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using D121906(SPHK2 Antibody) at dilution 1/60, on the right is treated with recombinant protein. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: RAMOS, LO2 cell lysates, Primary antibody: D121906(SPHK2 Antibody) at dilution 1/1600, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 5 seconds
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