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Order NO. D122232
兔抗DHCR7多克隆抗体
英文名 Anti-DHCR7 rabbit polyclonal antibody
别名 SLOS
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 DHCR7    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D122232-0025 25 ul 现货
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D122232-0100 100 ul 现货
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D122232-0200 200 ul 现货
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属性

Background: This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009] (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: DHCR7
Immunogen: Recombinant protein of human DHCR7
Full name: 7-dehydrocholesterol reductase
Synonyms: SLOS
SwissProt: Q9UBM7
ELISA Recommended dilution: 5000-10000
IHC positive control: Human liver cancer and Human gastric cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 54 kDa
WB Positive control: Caco-2, MCF7, Hela, LO2 cell, Mouse brain tissue lysates
WB Recommended dilution: 1000-5000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using D122232(DHCR7 Antibody) at dilution 1/80, on the right is treated with recombinant protein. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using D122232(DHCR7 Antibody) at dilution 1/80, on the right is treated with recombinant protein. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: Caco-2, MCF7, Hela, LO2 cell, Mouse brain tissue lysates, Primary antibody: D122232(DHCR7 Antibody) at dilution 1/1150, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 10 seconds
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