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Order NO. D123063
兔抗RPS3多克隆抗体
英文名 Anti-RPS3 rabbit polyclonal antibody
别名 S3
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 RPS3    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D123063-0025 25 ul 现货
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D123063-0100 100 ul 现货
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D123063-0200 200 ul 现货
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属性

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: RPS3
Immunogen: Fusion protein of human RPS3
Full name: ribosomal protein S3
Synonyms: S3; uS3
SwissProt: P23396
ELISA Recommended dilution: 5000-10000
IHC positive control: Human breast cancer
IHC Recommend dilution: 25-100
WB Predicted band size: 27 kDa
WB Positive control: HepG2, Jurkat, Hela, Raji, RAW264.7, A549, 293T cell lysates
WB Recommended dilution: 1000-5000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using D123063(RPS3 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)


  • Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-7: HepG2, Jurkat, Hela, Raji, RAW264.7, A549, 293T cell lysates, Primary antibody: D123063(RPS3 Antibody) at dilution 1/1000, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 20 seconds
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