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Order NO. D123289
兔抗TBP多克隆抗体
英文名 Anti-TBP rabbit polyclonal antibody
别名 HDL4; GTF2D; SCA17; TFIID; GTF2D1
相关类别 一抗 储存 冷冻（-20℃）
宿主 Rabbit 反应种属 Human
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 TBP    
编 号 规格 库存 目录价（￥） 您的价格（￥） 数 量
D123289-0025 25 ul 现货 待询价 待询价
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D123289-0100 100 ul 现货 待询价 待询价
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D123289-0200 200 ul 现货 待询价 待询价
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属性

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016] (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: TBP
Immunogen: Recombinant protein of human TBP
Full name: TATA-box binding protein
Synonyms: HDL4; TBP1; GTF2D; SCA17; TFIID; GTF2D1
SwissProt: P20226
ELISA Recommended dilution: 5000-10000
IHC positive control: Human esophagus cancer
IHC Recommend dilution: 50-100
WB Predicted band size: 38 kDa
WB Positive control: Hela, 293T cell lysates
WB Recommended dilution: 1000-5000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using D123289(TBP Antibody) at dilution 1/70, on the right is treated with recombinant protein. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hela, 293T cell lysates, Primary antibody: D123289(TBP Antibody) at dilution 1/1000, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 10 seconds