Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Applications:
ELISA, FC
Name of antibody:
CD27
Common name:
1F5, CDX-1127
Synonyms:
CD27;TNFRSF7;S152;T14;Tp55
SwissProt:
P26842
ELISA Recommended dilution:
5000-10000
FC Recommended dilution:
50-100
相关文档
Sample Application
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, hFc tagged protein (Catalog number: D145147) can bind Anti-CD27 monoclonal antibody (Catalog number: D146041) in a linear range of 3.2-80.0 ng/ml. In order to specifically detect D146041, mouse anti-human Fab-specific antibody was used as detection antibody.
HEK293 cell line transfected with human CD27 and irrelevant protein were surface stained with anti-CD27 monoclonal antibody (Catalog number: D146041) 1μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.