Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Applications:
ELISA, FC
Name of antibody:
CTLA-4
Common name:
BMS-734016, MDX-010, MDX-CTLA-4
Synonyms:
CTLA4;CD152
SwissProt:
P16410
ELISA Recommended dilution:
5000-10000
FC Recommended dilution:
50-100
相关文档
Sample Application
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CTLA4, hFc tagged protein (Catalog number: D145151) can bind Anti-CTLA-4 monoclonal antibody (Catalog number: D146045) in a linear range of 0.64-80.0 ng/ml. In order to specifically detect D146045, mouse anti-human Fab-specific antibody was used as detection antibody.
HEK293 cell line transfected with human CTLA4 and irrelevant protein were surface stained with anti-CTLA4 monoclonal antibody (Catalog number: D146045) 1μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.