ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human Trop2, mFc-His tagged protein (Catalog number: D145159) can bind Anti-Trop2 monoclonal antibody (Catalog number: D146046) in a linear range of 0.13-16.0 ng/ml.
Flow cytometry analysis of antigen binding of Anti-Trop2 monoclonal antibody (Catalog number: D146046). A clear peak shift of D146046 was seen compared to the control when incubated with Trop2-expressing A431 cells, indicating strong binding of D146046 to Trop2. Antibodies were incubated at 2 μg/mL.