ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD22, hFc-His tagged protein (Catalog number: D145009) can bind Anti-CD22 monoclonal antibody (Catalog number: D146054) in a linear range of 3.2-400 ng/ml. In order to specifically detect D146054, mouse anti-human Fab-specific antibody was used as detection antibody.
HEK293 cell line transfected with human CD22 and irrelevant protein were surface stained with Anti-CD22 monoclonal antibody (Catalog number: D146054) 1 μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.