ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human B7-H5 Protein, hFc Tag (Catalog number: D145504) can bind Anti-B7-H5 monoclonal antibody (Catalog number: D146113) in a linear range of 2.44–625.00 ng/mL. In order to specifically detect D146113, mouse anti-human Fab-specific antibody was used as detection antibody.
Flow cytometry analysis with 1 μg/mL Anti-B7-H5 monoclonal antibody (Catalog number: D146113) on Expi293 cells transfected with Human B7-H5 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).