ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human IL1B Protein, hFc Tag (Catalog number: D145441) can bind Anti-IL1B monoclonal antibody (Catalog number: D146118) in a linear range of 0.61–156.25 ng/mL. In order to specifically detect D146118, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis under cell membrane permeable condition with 1 μg/mL Anti-IL1B monoclonal antibody (Catalog number: D146118) on Expi293 cells transfected with Human IL1B protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).