ELISA plate pre-coated by 1 μg/mL (100 μL/well) Cynomolgus IL9 Protein, hFc Tag (Catalog number: D145610) can bind Anti-IL9 monoclonal antibody (Catalog number: D146121) in a linear range of 2.44–156.25 ng/mL. In order to specifically detect D146121, mouse anti-human Fab-specific antibody was used as detection antibody.
Flow cytometry analysis under cell membrane permeable condition with 1 μg/mL Anti-IL9 monoclonal antibody (Catalog number: D146121) on Expi293 cells transfected with Human IL9 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).