ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD142 Protein, hFc Tag (Catalog number: D145372) can bind Anti-CD142 monoclonal antibody (Catalog number: D146122) in a linear range of 2.93–187.50 ng/mL. In order to specifically detect D146122, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis of antigen binding of Anti-CD142 monoclonal antibody (Catalog number: D146122). A clear peak shift of D146122 was seen compared to the control when incubated with CD142-expressing Hela cells, indicating strong binding of D146122 to CD142. Antibodies were incubated at 5 μg/mL.