ELISA plate pre-coated by 5 μg/mL (100 μL/well) Human TSLP Protein, hFc Tag (Catalog number: D145274) can bind Anti-TSLP monoclonal antibody (Catalog number: D146128) in a linear range of 741–6667 ng/mL. In order to specifically detect D146128, mouse anti-human Fab-specific antibody was used as detection antibody.
Flow cytometry analysis under cell membrane permeable condition with 1 μg/mL Anti-TSLP monoclonal antibody (Catalog number: D146128) on Expi293 cells transfected with Human TSLP protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).