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Order NO. D161451
兔抗SMAD3多克隆抗体
英文名 Anti-SMAD3 rabbit polyclonal antibody
别名 LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 SMAD3    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D161451-0025 25 ul 现货
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D161451-0100 100 ul 现货
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D161451-0200 200 ul 现货
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属性

Background: The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3. (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: SMAD3
Immunogen: Synthetic peptide of human SMAD3
Full name: SMAD family member 3
Synonyms: LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436
SwissProt: P84022
ELISA Recommended dilution: 5000-10000
IHC positive control: Human liver cancer and Human lung cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 48 kDa
WB Positive control: 293T cell lysate
WB Recommended dilution: 1000-5000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using D161451(SMAD3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using D161451(SMAD3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: 293T cell lysate, Primary antibody: D161451(SMAD3 Antibody) at dilution 1/1000, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 30 seconds
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