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Order NO. D162896
兔抗GPC3多克隆抗体
英文名 Anti-GPC3 rabbit polyclonal antibody
别名 glypican 3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 GPC3    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D162896-0025 25 ul 现货
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D162896-0100 100 ul 现货
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D162896-0200 200 ul 现货
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属性

Background: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
Applications: ELISA, WB, IHC
Name of antibody: GPC3
Immunogen: Synthetic peptide of human GPC3
Full name: glypican 3
Synonyms: SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
SwissProt: P51654
ELISA Recommended dilution: 5000-10000
IHC positive control: Human thyroid cancer and Human bladder cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 66 kDa
WB Positive control: A172 cell lysate
WB Recommended dilution: 500-2000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using D162896(GPC3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human bladder cancer tissue using D162896(GPC3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: A172 cell lysate, Primary antibody: D162896(GPC3 Antibody) at dilution 1/900, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 40 seconds
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