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Order NO. D164690
兔抗PUF60多克隆抗体
英文名 Anti-PUF60 rabbit polyclonal antibody
别名 poly(U) binding splicing factor 60; FIR; VRJS; RoBPI; SIAHBP1
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 PUF60    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D164690-0025 25 ul 现货
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D164690-0100 100 ul 现货
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D164690-0200 200 ul 现货
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属性

Background: This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: PUF60
Immunogen: Synthetic peptide of human PUF60
Full name: poly(U) binding splicing factor 60
Synonyms: FIR; VRJS; RoBPI; SIAHBP1
SwissProt: Q9UHX1
ELISA Recommended dilution: 5000-10000
IHC positive control: Human cervical cancer and Human breast cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 60 kDa
WB Positive control: 293T, Hela, Mouse brain tissue, LOVO cell lysates
WB Recommended dilution: 1000-5000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using D164690(PUF60 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using D164690(PUF60 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: 293T, Hela, Mouse brain tissue, LOVO cell lysates, Primary antibody: D164690(PUF60 Antibody) at dilution 1/800, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 20 seconds
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