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Order NO. D190751
小鼠抗PCNA单克隆抗体
英文名 Anti-PCNA mouse monoclonal antibody
别名 proliferating cell nuclear antigen; ATLD2
相关类别 一抗 储存 冷冻(-20℃)
宿主 Mouse 反应种属 Human, Mouse, Monkey, Rat, Hamster
克隆类型 Mouse Monoclonal 抗原 PCNA
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D190751-0100 100 ul 预计5日内发货
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产品描述

概述

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

应用

免疫印迹(WB),免疫沉淀(IP),免疫荧光(IF),免疫组织化学(IHC)

属性

保存缓冲液 -20°C, PBS(pH 7.4) containing 0.02% sodium azide and 50% glycerol
运输条件 2-8°C
靶点 Uniprot 登记号 P12004
靶点基因 ID 5111
靶点全称 proliferating cell nuclear antigen
靶点别名 ATLD2
靶点研究领域 Epigenetics and Nuclear Signaling, Cancer
亚型 IgG2b
Sample Application

  • The image is immunofluorescence of Hela cells fixed in 100% methanol for 2h at -20°C using D190751(PCNA Antibody) at dilution 1/100.

  • The image is immunohistochemistry of paraffin-embedded Human colorectal carcinoma tissue using D190751(PCNA Antibody) at dilution 1/400. (Original magnification: ×200)

  • Immunoprecipitation analysis of Hela cell lysates using D190751(PCNA Antibody).

  • Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane 1-5: CHO-K1, C6, COS7, 3T3 and Hela cell lysates, Primary antibody: D190751(PCNA Antibody) at dilution 1/1000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 5 minutes
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