网 站
反 馈
网 站
指 南
Order NO. D190795
小鼠抗EIF2S1单克隆抗体
英文名 Anti-EIF2S1 mouse monoclonal antibody
别名 eukaryotic translation initiation factor 2 subunit alpha; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
相关类别 一抗 储存 冷冻(-20℃)
宿主 Mouse 反应种属 Human, Mouse, Rat
克隆类型 Mouse Monoclonal 抗原 EIF2S1
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D190795-0050 50 ul 预计5日内发货
加入购物车
D190795-0100 100 ul 预计5日内发货
加入购物车
产品描述

概述

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

应用

免疫印迹(WB),免疫荧光(IF),免疫沉淀(IP)

属性

保存缓冲液 -20°C, PBS(pH 7.4) containing 0.03% Proclin300 and 50% glycerol
运输条件 2-8°C
靶点 Uniprot 登记号 P05198
靶点基因 ID 1965
靶点全称 eukaryotic translation initiation factor 2 subunit alpha
靶点别名 EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
靶点研究领域 Epigenetics and Nuclear Signaling
亚型 IgG2b
Sample Application

  • The image is immunofluorescence of Hela cells fixed by anhydrous methanol for 2h at -20°C using D190795(EIF2S1 Antibody) at dilution 1/200.

  • Immunoprecipitation analysis of Hela cell lysates using D190795(EIF2S1 Antibody).

  • Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-7: K562, COS7, 3T3, C2C12, MCF-7, C6 and Hela cell lysates, Primary antibody: D190795(EIF2S1 Antibody) at dilution 1/1000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 5 minutes
    我的购物车(0) 结算